Authored by Mariana Georgieva Yordanova*
Abstract
Aim: To evaluate the status of oxidative stress and antioxidant systems (thiol and biological plasma antioxidant potential) in patients with heart failure, combined with disorders of carbohydrate metabolism and renal failure, and to what extent their values depend on the duration and severity of the disease (by NYHA).
Materials and Methods: Forty subjects (age 66.4±6.8) admitted for exacerbated chronic heart failure (III and IV FC), and 12 of them were examined in the same age range without clinical and echocardiography for HF. The levels of total thiols (SHp), reactive oxygen metabolites (dROMs), plasma antioxidant potential (BAP), creatinine, calculated GFR, glucose and C-reactive protein are analysed. Measurement of SHp, dROMs and BAP assays was performed spectrophotometrically with kits of Diacron International-Italy with an Olympus AU400 biochemical analyser and Carpe diam analyser (Diacron).
Results: An increase in oxidative stress was observed in the patient group, which had a high negative correlation with the thiol groups and the plasma antioxidant potential (r=-0.7404; r=-0.7567). The decrease in glomerular filtration also corresponds to a high degree with the reduction of the organism’s antioxidant capacity and the increase of oxidative stress. We found a high degree of dependence on the severity of HF (according to the NYHA classification), with the studied levels of oxidative stress and the antioxidant barrier. A similar relationship is observed between disease duration and SHp, dROMs and BAP concentrations.
Conclusion: Oxidative stress plays an essential role in the pathogenesis and progression of CHF. The observed thiol levels have decreased statistically significant, such as the collapse of the antioxidant system is key in the disturbed homeostasis of organisms in ROS/RNS coping.
Keywords: Thiols; Oxidative stress; Antioxidant system; Heart failure; GFR, Inflammatory response.
Introduction
Heart failure is a severe chronic condition where, as a result of dysfunction, the heart is unable to expel sufficient blood to meet the body’s metabolic needs. The most common causes are coronary heart disease, myocardial infarction, congenital heart defects, damage to the heart valves, atrial fibrillation. Heart failure (HF) is the most common cause of hospitalisation in people over 65 and is associated with increased health costs. It is a global pandemic affecting at least 26 million people worldwide, with disease rates increasing as the population ages [1]. The death rate from this diagnosis in Bulgaria has doubled, with over 20,000 people dying annually. Oxidative stress is a key element in the pathophysiology of heart failure (HF) [2]. Several studies support the hypothesis of a link between oxidative status change and the development and progression of many diseases, such as neurodegenerative conditions, cardiovascular, inflammatory diseases, and cancer [2-5]. In the course of oxidation processes, reactive free radicals (ROS) are formed continuously, which are missing one electron. To achieve their stability as compounds, they obtain electrons from other organic molecules such as DNA, fats, proteins, and enzymes by oxidising them. Free radicals are extremely reactive, damaging cell membranes and DNA molecules [6] (Figure 1).
Physiologically free radicals are formed during normal metabolic processes in the cell. They play a role in the regulation of basic functions and act as signalling molecules in the transduction cascades or pathways for various growth factors, cytokines and hormones [6]. On the other hand, the so-called “oxidative explosion” is absolutely necessary for the initiation of immunological responses. It activates neutrophil cells and phagocytosis as the body’s primary immune response against foreign antigens. Oxidative stress is a term used to refer to an imbalance between the concentrations of reactive oxygen and nitrogen species (RNS) formed and the body’s antioxidant protection. The body counteracts oxidative stress with its plasma and intracellular antioxidants. An antioxidant is any substance or compound that can bind ROS and RNS or inhibit the oxidation process in the cell. The deficiency of natural antioxidants increases with advancing age and the worsening of chronic pathological processes.
Thiols are compounds with a free sulfhydryl (R-SH) group. Most often they are presented in the form of proteins containing one or more free cysteine groups, or low molecular weight compounds (e.g. glutathione GSH). They are localised in cells and extracellular fluids. They are endogenous organic compounds that support the maintenance of aerobic cells in a reducing state despite the oxidising environment. R-SH exists in vivo in three forms, which include free thiol (e.g., cysteine), identical thiols formed between two homodisulfides (e.g., GSSG glutathione disulfide) and heterodisulfides formed between different thiols (e.g. proteinbound thiols). Proteins with their sulfhydryl groups are the main component of the serum antioxidant effect. In the case of high oxidative stress, thiol levels are reduced to neutralise reactive oxygen species. In this case, the sulfhydryl groups of the thiols are oxidised to form disulfide (-S-S-) bonds. These connections can be transformed back to thiols, and thus the thiol/disulfide homeostasis is maintained [7]. Thiol is one of the most important antioxidant barriers in humans, and thiol / disulfide homeostasis is a new marker for oxidative stress [8]. In a recent study, SH protein groups were found to contribute 52.9% of measured total serum antioxidant capacity in healthy subjects [9]. Impaired balance oxidative stress, antioxidant defences and changes occurring in the redox thiol system are associated with ischemic heart disease, atherosclerosis, renal and hepatic failure, rheumatoid arthritis, dementia and Alzheimer’s disease. In addition to their role in protecting against free radicals, thiols share a significant role in detoxification, signal transduction, apoptosis, and various other molecular-level functions.
Frenay A-RS, et al. shows that high serum concentrations of R-SH are associated with a favourable cardiovascular risk profile and better survival for patients with the transplanted kidney [10]. Oxidative stress causes the development of myocardial and vascular dysfunction and is an important pathophysiological factor in HF. Quantitative studies of reactive oxygen metabolites (dROMs), biological antioxidant potential (BAP), and the main aminothiol compounds in plasma or serum stimulate scientific search with the potential for therapeutic modulation.
Materials and Methods
Forty patients (24 men and 16 women) that have a mean age of 66.38 years (range 54-78 years) admitted to the MMAVarna Cardiology Department for exacerbated CHF (NYHA IIIIV FC) were examined. Criteria for inclusion in the study are the presence of typical subjective and objective symptoms of heart failure and ultrasound-proven ventricular dysfunction (ejection fraction <35%). The control group consisted of 12 individuals with a mean age of 62.5 years (range 55-70 years) with no subjective and objective data on cardiovascular disease, no clinical and laboratory criteria for diabetes and impaired renal function. The study was approved by the Ethics Committee of MMA-Varna. Body mass index (BMI) was determined in both groups. A standard pre-analytical procedure is used to take venous blood. The blood serum was removed by centrifugation for 10 minutes at 3500 rpm. In serum, we determine the following indicators: creatinine, CRP, glucose, cholesterol, oxidative stress (dROMs), serum antioxidant capacity (BAP), and total serum thiol. GFR was calculated based on serum creatinine using the MDRD formula. Oxidative stress (dROMs) and serum antioxidant capacity (BAP) were determined spectrophotometrically with ready-made kits from Diacron International-Italy using a semi-automatic Carpe diam analyser (Diacron Labs Italy). The d-ROM test is based on the ability of a biological sample to oxidise aromatic amine (DPPD). Reactive oxygen metabolites (ROMs) (hydroperoxides, chloramines and their derivatives) react with appropriately buffered chromogen and develop a colour derivative that is photometric at 505 nm. The concentration of this class of ROMs directly correlates with the intensity of the colour and the increased levels of oxidative stress. The units that express dROMs are Carratelli units (1 CARR U=0.08 mg% hydrogen peroxide). The range in healthy individuals is 250- 300 UCARR.
The BAP test is spectrophotometric (λ=505 nm) for measuring the antioxidant capacity (AOC) of a sample placed in a solution of ferric chloride. It has the ability to reduce iron from ferric Fe3+ to Ferro Fe2+ ion. Reducing the intensity of the colour is a criterion for the concentration of antioxidants present in the plasma. For both tests, quality control is performed with two levels of control materials (Low and High).
The testing of plasma thiol groups (R-SH) (lipoic acid, glutathione, cysteine) is performed with a colourimetric SHp test (Diacron International-Italy) with an Olympus AI 400 biochemical analyser with our adapted program. The principle of the method is based on the interaction of thiols with 5, 5’-dithiobis- (2-nitrobenzoic acid) (DTNB), forming highly coloured anions with maximum absorption at 405 nm. Thiols are extremely unstable. Within the same day, samples were examined in fresh plasma (serum) without a trace of hemolysis. Pre-analytic requirements have been described and imposed because time and oxygen from the air enhances the reduction of glutathione by γ-glutamyl cysteine and the rapid conversion of reduced glutathione into oxidised form, which interferes with the result. Each series was controlled by two levels of controls - low and high. VK 2.8%; 5.2%, bis 2.8% respectively for low control and high. CRP and serum creatinine were determined using routine methods of an Olympus AU 640 biochemical analyser with Beckman Coulter reagents (USA).
Statistical Methods
Data analysis is performed with Graph Pad Prism v software. 6.0 using standard statistical methods (descriptive statistics, nonparametric T-test for mean comparison, ANOVA test for continuous variables with normal distribution between the groups of analysis, variance and Pearson correlation analysis). Biochemical parametric data were presented as mean and standard deviation (mean±SD) at a 95% confidence interval. Statistical significance was stated at p <0.05.
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