Authored by Sidaoui Abouamama*,
Abstract
The 20 isolates of Fusarium oxysporum f. sp. albedinis (Foa) produce amylase and cellulase enzymes, with the development of lysis zones around the inoculum (clear region) on the selective medium, indicates starch hydrolysis and cellulose degradation, with a variation in the ability of the isolates to produce these two enzymes, and this after measuring the diameters of the lysis zone. After Principal Component Analysis (PCA), we found that there is a strong positive correlation between mortality rate and amylolytic activity CP1 (74.80%) on the one hand and a weak correlation with cellulase CP2 (22.3%), on the other hand. While, there is no correlation between the pathogenicity of the isolates and the geographical origin of the isolate or cultivar from which this isolate was isolated P=0.3042.
Keywords: Amylase; Cellulase; Fusarium oxysporum f. sp. albedinis
Introduction
The blessed tree mentioned in the Qur’an, symbol of life in arid and semi-arid regions, The date palm Phoenix dactylifera L. is a monocotyledon dioecious plant, belongs to the family of Arecaceae [1-3]. Which includes 200 genera and more than 2500 species, this plant is cultivated for more than 4000 years, and the genus includes about 14 species distributed in the desert, tropical and subtropical zone of the globe [4-6]. Date palm has long been recognized as one of the most important resources of arid and saharan habitats, because they represent an ecological, economic and social importance considerable for many countries in the arid zones, which are among the poorest in the world. Indeed, date palms widely cultivated for: first, its multiple uses and its ecosystem services, especially for its comestibles fruits and constitute the principal financial resources of oasis cultivators, and second, for its ability to adapt to the conditions of the most severe arid climates, who contributes to the development of subjacent (cereals, fodder, vegetables, tomato, saffron and fruit trees cultures), constitute the main axis of agriculture in the desert regions and allowing the development of various forms of animal and plant life essential for the maintenance and survival of desert populations [7,2,8].
In Algeria, date palm Phoenix dactylifera L . is the essential component of the oasis ecosystem. It is cultivated at the level of 16 provinces with a surface of 120830 hectares, Thus, Algeria is ranked 4th in the world and first in the Maghreb, with about 18,605,100 palms, whose productive potential is 9,641,680 tons. In these regions, palm is considered as fortune of the most important economic resources, and this offers numerous benefits to the population of desert areas, especially in social terms. However, in recent years the date production has experienced a significant decline principally due to vascular fusariosis, known locally as Bayoud caused by a soil fungus Fusarium oxysporum f. sp. albedinis (Foa) [3]. It is considered one of the most serious diseases, which poses a permanent threat to the palm groves of North Africa and leads to the disappearance of 20 million palm trees, including three million Algerian palm trees, ever since 1870 [8-10]. In most of the infested oases, the Bayoud has made disappeared the best variety such as Boufeggous, Medjhoul in Morocco and Deglet Nour in some oases in the south of Algeria [11].
The objective of this work is to study the correlation between the activity of hydrolytic enzymes and the pathogenicity of some isolates of Fusarium oxysporum f. sp. albedinis on the one hand, and the correlation between the geographic origin and the pathogenicity of these isolates on the other hand.
Material and Methods
Isolation of pathogenic fungi
The isolates of Foa used in this study ware isolated in our previous work [12,13].
Amylase activity of Fusarium oxysporum f.sp. albedinis
The search for the amylolytic activity of our isolates was carried out on a starch-based medium, according to the method described by [14]. An explant of 0.5 cm of diameter of each isolate was deposited in the center of a Petri dish containing the PSA medium at pH 5, supplemented with 1% soluble starch. The dishes are then incubated at 30 °C. for 24 to 72 hours. After incubation, a solution of lugol was sprayed on the surface of the dich, the background of the medium appears blue and clear halos appear around of the colony, witnesses an amylolytic activity [15-17]. The diameter of the lysis zones was measured for the three repetitions that have been performed.
Cellulosic activity of Fusarium oxysporum f.sp. albedinis
The most reliable technique for demonstrating cellulase activity is to show the ability of isolates to assimilate cellulose, on a solid medium based on cellulose, whose composition is as follows: NaNO3 (2g), K2HPO4 (1g), MgSO4, H2O (1g), KC (10.5g), ZnSO4, H2O (0.005g), CuS4, 5H2O (0.001g), MnCl2 (0.001g), Cellulose (Carboxyl), methylcellulose) (2.5g), agar (30g), 1000 ml of distilled water, pH = 5 [18,19]. For this we deposited an explant of 0.5 cm of diameter of each isolate in the center of a Petri dish containing the cellulosebased medium and incubated at 30 °C for 2 to 3 days. After incubation, a solution of Congo Red was then poured into each Petri dish; the dishes were then treated with a flooding with 1M NaCl for 15 minutes. The degradation of the cellulose is visualized after the appearance of a clear zone around of the fungal colony [15,16,20].
Correlation between pathogenicity and the two enzymes
Correlation between pathogenicity and amylase and cellulase was analyzed by the analysis of Principal Component Analysis (PCA) by the JMP software (SAS Pro (JMP®, Version <12>. SAS Institute Inc., 2015). The principle of this method was to find the axes that best represent the correlation of the isolates in the multidimensional space of the three parameters (mortality rate, amylase and cellulase).
Histological studies
The induction of the disease and the reproduction of the symptoms attest to the belonging of the inoculated isolates to the special form albedinis . At the end of the trial, and to confirm the infection of the young seedlings by Foa, we considered it useful to carry out transverse and longitudinal histological sections of the roots after a month of inoculation.
Results
Amylase activity of Fusarium oxysporum f.sp. albedinis
The 20 isolates were developed lysis zones (clear region) on the selective medium, indicated the hydrolysis of the starch, with varied amylolytic activity between the isolates after the measurement of the diameter of the zones formed (Figure 1 & Table 1).
Table 1: Diameters of amylolytic activity of Foa.
D1-D3: Diametre (cm)
M%: Mortality rate in %
Cellulosic activity of Fusarium oxysporum f.sp. albedinis
Hydrolytic enzymatic activity showed that all Foa isolates were able to produce cellulose, the cellulose degradation after incubation was observed by the development of lysis zones (light halo) on the selective medium. We noted that there is variation in the ability of isolates to produce cellulase after measuring the diameters of the lysis zone (Figure 2 & Table 2).
Table 2: Diameters of Cellulosic activity of Foa.
D1-D3: Diametre (cm)
M%: Mortality rate in %
Histological studies
Our microscopic observations on the anatomy and morphology as well as, the histochemical reactions in the roots of the seedlings palms, firstly made it possible to highlight and confirm the characteristics of the anatomical structures of the roots, and then showed that the periphery of these roots was characterized by brown spots, resulting accumulation of phenolic compounds occurred in date palm callus in response to the infection by Foa. The secretion of polyphenols represents an important role in the resistance process against Foa (Figure 3 & Table 3).
Table 3: Main components calculated with correlations (proper values).
Correlation between the two enzymes, geographical origin and the pathogenicity
We noted that there is a strong positive correlation between mortality rate and amylolytic activity and low correlation with cellulose. Our results are explained by two main components (Figure 4), the first main component CP1 (74, 80%), is more correlated with mortality rate and amylase activity (Table 3), this suggests that these two variables vary together if one increases, the other tends to increase. This CP1 can be considered as a measure of the mortality rate (0, 63) and the amylase activity (0, 62) (Table 4). The second main component CP2 (22, 3%), is strongly correlated with cellulase activity (0, 89) (Figure 4), it only augment with the augmentation in cellulase activity, This CP2 can be considered as a measure of cellulase activity (Figure 4 & Table 4).
Table 4: Main components calculated with correlations (proper vectors).
Levels not connected by same letter not significantly different at P=0, 05 so there is no correlation between the pathogenicity of the isolates and the geographical origin of this isolates (Table 5).
Table 5: Student’s t Test.
Discussion
Pathogenicity testing is the only way to determine the pathological effect of fungal strains present in diseased plants or in soil samples [21], this pathogenicity in the species Fusarium oxysporum led to the definition of special forms theoretically subservient, each with a single host plant, these forms are morphologically identical, but sometimes have very narrow parasitic specificity, each has a single host plant, these forms are morphologically identical, but sometimes have a very narrow parasitic specificity, unfortunately in the species Fusarium oxysporum, the variability of pathogenicity has been little studied [22, 23].
Currently, the identification of pathogenic Fusarium oxysporum isolates is mainly based on pathogenicity tests [24]. In our work the high mortality rate was recorded in seedlings inoculated with the 20 isolates of Foa, result confirming their belonging to the special form albedinis , compared to control seedlings, these results also coincide with those reported by several authors who mention the pathogenicity of Foa isolates against date palm seedlings [25-27].
The results obtained showed that there is no correlation between the pathogenicity of the isolates and the geographical origin of the isolate or cultivar from which this isolates were isolated, this result is similar to that reported by [28]. In addition, [29] reported that Fusarium species isolated from some parts of Europe were not able to cause the same levels of infection under different agroecological conditions.
The observation of brown necrosis in the roots of seedlings palms after inoculation, it’s an indicator of the passage of Foa mycelium in vascular tissues, and these results correspond to those published by [30]. We then observed that the periphery of these roots was characterized by brown spots, resulting in the secretion of phenolic compounds against Foa, and this after the realization of the histological sections. Knowing that, the phenolic compounds involved in Bayoud resistance are documented [31, 32].
Studies related to the production of enzymes by a phytopathogenic fungus are complicated, particularly because of the presence of plant enzymes and microbial enzyme inhibitors, which occur in plants. Indeed, the enzymatic activity was tested for the first time by [33]. Currently, the most practical way to study the production of enzymes by a fungus is to study their production on artificial growth media, which contain no inhibitors [34].
Phytopathogenic fungi are responsible for significant plant damage and cause greater yield losses than bacteria and viruses [35], due to wide range of hydrolytic enzymes secreted during host plant infection, which will allow fungal cells to enter host plant cells by breaking down cell walls and causing pathogenesis in the host plant. It’s the case, for example, of the species Fusarium oxysporum which excretes cellulases responsible for the hydrolysis of the plant cell wall [19,36].
In our work, it was noted that all Foa isolates produce cellulase and amylase, with the development of a clear zone around the isolates, production vary between isolates depending on the diameter of the lysis zone. This result is comparable to those published by [15], and that of [20], which reported that 81% of fungal strains produce cellulase and 66.7% produce amylase.
Conclusion
After our study, our results showed the absence of correlation between the geographical origin and the pathogenicity of the isolates. As well, all this isolate was able to produce the hydrolytic enzymes on selective media used, with a strong positive correlation between mortality rate and amylolytic activity and a weak correlation with cellulase.
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