Scutellaria Barbata Flavonoids Regulate Protein Phosphorylation of Glycogen Synthase Kinase-3β and Tau in N2a Cells with Aβ 25-35-Induced Neurotoxicity Alzheimers - Complimentary & Alternative Medicine 

Alzheimer’s disease (AD) is a neurodegenerative disease caused by chronic or progressive  structural  damage of the brain. Neurofibrillary tangles (NFTs) induced by excessive phosphorylation of tau protein have toxity to neurons and to be one of the main pathogenesis of AD. Then, inhibiting the excessive phosphorylation of Tau protein is the key point to treat AD. While glycogen synthase kinase-3β (GSK-3β) plays an important role in causing abnormal hyperphosphorylation of AD-like Tau protein. It has been confirmed that GSK-3β can catalyze the hyperphosphorylation of Tau protein at several sites. Therefore, we research the regulatory effects of Scutellaria barbata flavonoids (SBF) on β-amyloid25-35 (Aβ25-35) induced Tau phosphorylation at Ser202, Ser199, Ser404, Ser214, Thr231 sites and regulatory mechanism by GSK-3β in mouse brain neuroblastoma cells(N2a). Our results showed that compared with control group, the protein expressive level of p-Tau (Ser202), p-Tau (Ser199), p-Tau (Ser404), p-Tau (Ser214) and p-GSK-3β (Tyr216) in model group were higher than those of control group (p<0.05, p<0.01).

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