Authored by Alexandrov MT
Aim: In vitro using Raman-fluorescence spectroscopy study the effect of saliva and hygiene products for oral cavity on the mineralization indicators of various anatomical and topographic zones of the teeth for their various functional groups, justify its clinical expediency and effectivity.
Methods: In preclinical In vitro study on 90 model tooth test objects (incisors, premolars and molars) removed according to clinical indications, Raman-Fluorescence study of the degree of mineralization (Raman characteristics spectrum) was conducted and violation of the hygienic condition of the teeth (the presence of plaque and its fluorescence intensity) in various functional groups of teeth was also investigated. For the registration of the studied parameters APK “InSpectr M” with a wavelength probe radiation of 532 nm was used. Advantages of Raman Fluorescent spectroscopy for determining the degree of mineralization and hygienic condition tooth digital tissues are objectivity (digital technology), expressivity, non-invasive, simple and non-destructive degree control of mineralization / demineralization of the selected tooth tissues and its hygienic state, possibility of documenting and storing information (creating a database).
Result: During the study a qualitative and quantitative analysis of the effects of saliva and hygiene products for the oral cavity on mineralization and hygienic condition of various functional groups of teeth was performed. Currently, dental caries is one of the most common dental diseases among children and adults of the Russian Federation. One of local factors of caries occurrence are unsatisfactory hygienic oral care and changes in the quantitative and qualitative composition of the oral fluid [1]. In the system of caries prevention, the leading aspect is oral hygiene. The theoretical justification for the use of oral hygiene with the aim of remineralization in the prevention of caries and mineralization of hard tooth tissues are scientific studies confirming that the most important feature of enamel is permeability, which is ensured by the presence of micro-spaces in it, filled with water, which are able to permeate substances both inorganic and organic nature - depending on their polarity and size. Penetration and the movement of ions in the aqueous phase of the enamel is promoted by osmotic pressure, which is the main mechanism of the process of remineralization and demineralization of solid tooth tissues. In this physiological process, the decisive role is played by the oral fluid (saliva), which is the main source of income of substances in tooth structure [2-4]. However, the unexplored question of how to expressly, simultaneously and without changing the studied structures of the tooth hold the diagnostic measurements of its mineralization and the presence of plaque, and how oral hygiene products (toothpastes and powders of different mechanism of action) and methods of their use affect the mineralization of hard tooth tissues and their hygienic status.
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